A replicative recombinant HPV16 E7 expression virus upregulates CD36 in C33A cells
نویسندگان
چکیده
Objective In past decades, the role of high-risk HPV (HR-HPV) infection in cancer pathogenesis has been extensively studied. The viral E7 protein expressed pre-malignant cells identified as an ideal target for immunological intervention. However, cultivation vitro remains a significant challenge, well lack methods expressing and generating replication-competent recombinant particles, which posed major obstacle to further exploration function carcinogenic mechanisms oncoprotein. Therefore, it is imperative investigate novel methodologies construct particles that express facilitate study its function. Methods We initiated construction by utilizing ccdB-Kan forward/reverse screening system conjunction with Red/ExoCET system. followed C33A obtained virus enable continuous expression HPV16 E7. Afterwards, total RNA was extracted performed transcriptome sequencing using RNA-Seq technology identify differentially genes associated HPV-induced oncogenicity. Results successfully established replicative stably continuously. were infected achieve overexpression protein. Subsequently, analysis conducted assess changes host cell gene expression. results revealed upregulation CD36 gene, oncogenic pathways, including PI3K-Akt p53 signaling pathway. qRT-PCR due Conclusion successful demonstrates replicated retains replication abilities Ad4, while also upregulating involved thereby promoting proliferation. outcome this provides perspective serves solid foundation HPV-related carcinogenesis development vaccines capable inducing protective immunity against HPV.
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ژورنال
عنوان ژورنال: Frontiers in Microbiology
سال: 2023
ISSN: ['1664-302X']
DOI: https://doi.org/10.3389/fmicb.2023.1259510